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桂林地区乙肝患者HBV核苷类似物耐药位点分析

苏何玲;班亚珂;左晓臣;徐东平;朱华;莫之婧;刘青波;刘永明

【期刊名称】《实用医学杂志》 【年(卷),期】2012(028)019

【摘要】检测桂林地区乙型肝炎患者体内HBV逆转录酶基因的耐药突变情况,为制订合理的治疗方案提供依据.方法:提取乙肝患者血清中HBV DNA,巢式PCR扩增其逆转录酶基因后测序,Vector NTI Suite 8.0及chromaslite201分析测序结果,Mega4.0进行基因分型.结果:171例患者测序结果中18例发现有基因耐药突变,其中rtA181位点突变7例,占突变总数的38.9％.多药耐药基因突变12例,点突变总数的66.7％.B基因型患者耐药突变率为10.4％,C基因型患者耐药突变率为10.8％,两基因型分布比率的差异无统计学意义(P＞0.05).结论:桂林乙肝患者HBV逆转录酶基因耐药突变组合较为复杂,多药耐药基因突变比率较高.耐药突变的HBV基因型分布比率在所检测患者中无明显差异.%Objective To investigate the hepatitis B virus (HBV) drug-resistant mutations against nucleos (t)ide analogues of the patients with chronic hepatitis B in Guilin region for designing more effective therapeutic strategies. Methods Serum HBV DNA was extracted and a nested PCR was employed for HBV reverse transcriptase gene amplification.Direct sequencing of PCR product was performed and the results were analyzed by Vector NTT Suite 8.0 and chromaslite201 tools.Then the viral genotype was identified after phylogenetic analysis by Mega4.0 software. Results Eighteen patients were detected to be drug-resistant