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1. enzyme engineering
2. What is microorganism fermentation production for enzyme?
3.Why microorganism should be used in the production of enzyme preparations?? 4.Process of enzyme biosynthesis
synchronous synthesis medium-term synthesis continuation synthesis Lagging synthesis 4. Enzyme induction Enzyme repression 5.
? Western blotting ? ELISA Indirect Sandwich Competitive
? Protein microarry 6.ribozyme
Self splicing
I intron 和II intron
self cleavage
hammerhead structured 、hairpin、annularity -HDV和RNaseP 7.Abzyme
An example of an example of the application of an antibody enzyme? A case study of the preparation of an antibody enzyme? The characteristics of the antibody enzyme?
Similarities and differences between antibodies and enzymes? artificial enzyme
Blotting and artificial enzyme? 8.Immobilized Enzyme
properties of immobilized enzyme?
Table: Comparison of the Characteristics of Different Methods of Enzyme Immobilization
9.Directed Evolution
Compared with the conventional DNA Shuffing technology, the characteristics of RPR Technology?
what is Stagger extension process?
random-priming in vitro recombination, RPR DNA shuffling
Compared with conventional mutation techniques? 10.
Dialysics
Chromatography
Gel filtration Chromatography
Ion-Exchange Chromatography
Affinity Chromatography
Hydrophobicity Chromatography
Isoelectric focusing
Electrophoresis