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1. enzyme engineering

2. What is microorganism fermentation production for enzyme?

3.Why microorganism should be used in the production of enzyme preparations?？ 4.Process of enzyme biosynthesis

synchronous synthesis medium-term synthesis continuation synthesis Lagging synthesis 4. Enzyme induction Enzyme repression 5.

? Western blotting ? ELISA Indirect Sandwich Competitive

? Protein microarry 6.ribozyme

Self splicing

I intron 和II intron

self cleavage

hammerhead structured 、hairpin、annularity -HDV和RNaseP 7.Abzyme

An example of an example of the application of an antibody enzyme？ A case study of the preparation of an antibody enzyme？ The characteristics of the antibody enzyme？

Similarities and differences between antibodies and enzymes？ artificial enzyme

Blotting and artificial enzyme？ 8.Immobilized Enzyme

properties of immobilized enzyme？

Table: Comparison of the Characteristics of Different Methods of Enzyme Immobilization

9.Directed Evolution

Compared with the conventional DNA Shuffing technology, the characteristics of RPR Technology？

what is Stagger extension process?

random-priming in vitro recombination, RPR DNA shuffling

Compared with conventional mutation techniques？ 10.

Dialysics

Chromatography

Gel filtration Chromatography

Ion-Exchange Chromatography

Affinity Chromatography

Hydrophobicity Chromatography

Isoelectric focusing

Electrophoresis