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C600 hflA150 (Y1073, BNN102)

F- thi-1 thr-1 leuB6 lacY1 tonA21 glnV44 λ- hflA150(chr::Tn10)

host for repressing plaques of λgt10 when establishing cDNA libraries ? Reference Young R.A. and Davis, R. (1983) Proc. Natl. Acad. Sci. USA

80, 1194.

? Tetracycline resistance from the Tn10 insertion

?

CSH50

F- λ- ara Δ(lac-pro) rpsL thi fimE::IS1

See CGSC#8085

? References: Miller, J.H. 1972. Expts.in Molec.Genetics, CSH 0:14-0;

Blomfeld et al., J.Bact. 173: 5298-5307, 1991.

?

D1210

HB101 lacIq lacY+

DB3.1

F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1

useful for propagating plasmids containing the ccdB operon. ? gyrA462 enables ccdB containing plasmid propagation ? streptomycin resistant

? appears to NOT contain lacI (based on a colony PCR) --Austin Che

16:16, 18 June 2007 (EDT)

?

1. Bernard P and Couturier M. . pmid:1324324. PubMed HubMed

[Bernard-JMolBiol-1992] 2. Miki T, Park JA, Nagao K, Murayama N, and Horiuchi T. . pmid:1316444. PubMed HubMed [Miki-JMolBiol-1992] All Medline abstracts: PubMed HubMed

DH1

endA1 recA1 gyrA96 thi-1 glnV44 relA1 hsdR17(rK- mK+) λ- parent of DH5α

? An Hoffman-Berling 1100 strain derivative (Meselson68)

?

more efficient at transforming large (40-60Kb) plasmids ? nalidixic acid resistant

? Reference: Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.

?

DH5α

F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ–

An Hoffman-Berling 1100 strain derivative (Meselson68) ? Promega also lists phoA ? nalidixic acid resistant ? References:

o FOCUS (1986) 8:2, 9.

o Hanahan, D. (1985) in DNA Cloning: A Practical Approach

(Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia. o Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87:

4645-4649 PMID 2162051.

o Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.

?

DH5α Turbo (NEB)

F′ proA+B+ lacIq ? lacZ M15/ fhuA2 ?(lac-proAB) glnV gal R(zgb-210::Tn10)TetS endA1 thi-1 ?(hsdS-mcrB)5

? ? ? ? ?

Also known as NEB Turbo T1 phage resistant

Rapid growth: visible colonies on agar, ~6.5 hours; shaking liquid culture OD 600 = 2.0, ~4 hours Expresses the Lac repressor References:

o New England Biolabs, product catalogue number C2984H

DH10B (Invitrogen)

F- endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-

suitable for cloning methylated cytosine or adenine containing DNA ? an MC1061 derivative (Casadaban80). Prepare cells for chemical

transformation with CCMB80 buffer ? blue/white selection

?

While DH10B has been classically reported to be galU galK, the

preliminary genome sequence for DH10B indicates that DH10B (and by their lineage also TOP10 and any other MC1061 derivatives) is actually galE galK galU+. Dcekiert 16:37, 23 January 2008 (CST)

+

? Genome sequence indicates that DH10B is actually deoR. Presumably

TOP10 and MC1061 are also deoR+. ? Streptomycin resistant ? References:

o Casdaban, M. and Cohen, S. (1980) J Mol Biol 138:179 PMID 6997493.

o Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87:

4645-4649 PMID 2162051.

o E. coli Genetic Stock Center, MC1061 Record

o DH10B Genome Sequencing Project, Baylor College of Medicine o Complete sequence is available, see Durfee08, PMID 18245285.

?

DH12S (Invitrogen)

mcrA Δ(mrr-hsdRMS-mcrBC) φ80d lacZΔM15 ΔlacX74 recA1 deoR Δ(ara, leu)7697 araD139 galU galK rpsL F' [proAB+ lacIqZΔM15]

host for phagemid and M13 vectors

? useful for generating genomic libraries containing methylated cytosine

or adenine residues ? streptomycin resistant

? References: Lin, J.J., Smith, M., Jessee, J., and Bloom, F. (1991)

FOCUS 13, 96.; Lin, J.J., Smith, M., Jessee, J., and Bloom, F. (1992) BioTechniques 12, 718.

?

DM1 (Invitrogen)

F- dam-13::Tn9(CmR) dcm- mcrB hsdR-M+ gal1 gal2 ara- lac- thr- leu- tonR tsxR Su0

Host for pBR322 and other non-pUC19 plasmids; useful for generating plasmids that can be cleaved with dam and dcm sensitive enzymes ? Chloramphenicol resistant ? Promega lists as F' not F-

? Reference: Lorow-Murray D and Bloom F (1991) Focus 13:20

?

E. cloni(r) 5alpha (Lucigen)

fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

?

Common cloning strain.

E. cloni(r) 10G (Lucigen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA Common cloning strain. ? Resistant to phage T1.

?

E. cloni(r) 10GF' (Lucigen)

[F′ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA

?

Strain for cloning and single-strand DNA production.

E. coli K12 ER2738 (NEB)

F′proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10(TetR)/ fhuA2 glnV Δ(lac-proAB) thi-1 Δ(hsdS-mcrB)5

Phage propagation strain

? Also available from Lucigen Corporation.

?

ER2566 (NEB)

F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] Host strain for the expression of a target gene cloned in the pTYB vectors.

? Carry a chromosomal copy of the T7 RNA polymerase gene inserted

into lacZ gene and thus under the control of the lac promoter. In the absence of IPTG induction expression of T7 RNA polymerase is suppressed by the binding of lac I repressor to the lac promoter. ? Deficient in both lon and ompT proteases.

?

ER2267 (NEB)

F′ proA+B+ lacIq Δ(lacZ)M15 zzf::mini-Tn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14-(McrA-) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10

?

Commonly used for titering M13 phage because of the strain's F'

plasmid, which carries KanR, and its slow growth, which promotes easy visualization of plaques.

HB101

F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-

Please note that different sources have different genotypes so treat this information with caution.

? ? ? ? ? ? ?

From a GIBCO BRL list of competent cells.

Hybrid of E. coli K12 and E. coli B (but 98% K strain AB266 according to Smith et al.)

Host for pBR322 and many plasmids

Sigma lists the deletion Δ(gpt,proA). Check this. Promega does not list F-, mcrB, or mrr Streptomycin resistant References:

o Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41,

459.

o Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56 - pdf version from Invitrogen

o Lacks S and Greenberg JR (1977) J Mol Biol 114:153.

HMS174(DE3)

F- recA1 hsdR(rK12- mK12+) (DE3) (Rif R)

?

HMS174 strains provide the recA mutation in a K-12 background. Like BLR, these strains may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

DE3 indicates that the host is a lysogen of lDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

?

High-Control(tm) BL21(DE3) (Lucigen)

F– ompT gal dcm hsdSB(rB- mB-) (DE3)/Mini-F lacIq1(Gentr)

?

The HI-Control BL21(DE3) cells contain a single-copy BAC plasmid harboring a specially engineered version of the lacIq1 repressor allele.