鐩綍
鏇存柊
2011-6-7
绗?/p>
椤?/p>
鍏?/p>
11
椤?/p>
1
鍒嗗瓙鐢熺墿瀛﹀父鐢ㄨ瘯鍓傜殑閰嶅埗
........................................................................................
3
1
锛?/p>
LB(Luria-Bertani)
鍩瑰吇娑层�佸钩鏉跨殑閰嶅埗
.........................................................
3
2
锛庣惣鑴傜硸鍑濊兌鐨勯厤鍒?/p>
...........................................................................................
3
3
锛?/p>
P1
銆?/p>
P2
銆?/p>
P3
鐨勯厤鍒?/p>
P1
鐨勯厤鍒?/p>
.
.....................................................................
3
4
锛庡父鐢ㄧ紦鍐叉恫
.......................................................................................................
3
TE
.
....................................................................................................................
3
STE(
浜︾О
TEN)
..............................................................................................
3
STET
................................................................................................................
4
TNT
.
.................................................................................................................
4
Tris-
涔欓吀锛?/p>
TAE
锛?/p>
..........................................................................................
4
Tris-
纾烽吀锛?/p>
TPE
锛?/p>
.
..........................................................................................
4
Tris-
纭奸吀锛?/p>
TBE
锛?/p>
..........................................................................................
4
纰辨�х紦鍐叉恫
......................................................................................................
4
Tris-
鐢樻皑閰?/p>
.
.....................................................................................................
4
30
锛呬笝鐑叞鑳?/p>
..................................................................................................
4
10mol/L
涔欓吀閾碉細
.
.........................................................................................
4
1mol/LCaCl
2
....................................................................................................
5
2.5mol/LCaCl
2
.................................................................................................
5
1mol/L
浜岀～鑻忕硸閱囷紙
DTT
锛?/p>
锛?/p>
......................................................................
5
0.5mol/LEDTA(pH8.0)
锛?/p>
.
...............................................................................
5
婧村寲涔欓敪锛?/p>
10mg/ml
锛?/p>
锛?/p>
.................................................................................
5
1mol/LMgCl
2
锛?/p>
...............................................................................................
5
閰氾細姘豢
..........................................................................................................
5
纾烽吀缂撳啿鐩愭憾娑诧紙
PBS
锛?/p>
..............................................................................
5
涔欓吀閽炬憾娑诧紙鐢ㄤ簬纰辫瑙ｏ級
..........................................................................
5
3mol/L
涔欓吀閽?/p>
(pH5.2
鍜?/p>
7.0) ......................................................................
5
1mol/LTris
.
.......................................................................................................
5
Tris
缂撳啿鐩愭憾娑诧紙
TBS
锛?/p>
.
.............................................................................
5
5mmol/LNH4Ac
婧舵恫
....................................................................................
6
10mg/mlBSA
锛堝皬鐗涜娓呯櫧铔嬬櫧锛夋憾娑?/p>
.......................................................
6
10%
鍗佷簩鐑峰熀纭吀閽狅紙
SDS
锛?/p>
.
......................................................................
6
瀹為獙瀹ゅ父鐢ㄨ串瀛樻恫鐨勯厤鍒跺弬鏁?/p>
....................................................................................
7
1
锛?/p>
30%
涓欑儻閰拌兒婧舵恫
..........................................................................................
7
2
锛?/p>
40%
涓欑儻閰拌兒
..................................................................................................
7
3
锛庢斁绾胯弻绱?/p>
D
婧舵恫
.
.............................................................................................
7
4
锛?/p>
0.1mol/L
鑵鸿嫹涓夌７閰革紙
ATP
锛夋憾娑?/p>
.
..............................................................
7
5
锛?/p>
10mol/L
涔欓吀閰版憾娑?/p>
.......................................................................................
8
6
锛?/p>
10%
杩囩～閰搁摰婧舵恫
..........................................................................................
8
7
锛?/p>
BCIP
婧舵恫
.
........................................................................................................
8
8
锛?/p>
2脳
BES
缂撳啿鐩愭憾娑?/p>
.........................................................................................
8
9
锛?/p>
1mol/L CaCl2
婧舵恫
..........................................................................................
8
10
锛?/p>
2.5mol/L CaCl2
婧舵恫
....................................................................................
8
11
锛?/p>
1mol/L
浜岀～鑻忕硸閱囷紙
DTT
锛夋憾娑?/p>
.
...............................................................
8
12
锛庤劚姘ф牳鑻蜂笁纾烽吀锛?/p>
dNTP
锛夋憾娑?/p>
.
..................................................................
8